Identification of the Low Molecular Weight Copper Protein from Copper-intoxicated Mung Bean Plants.

نویسندگان

  • C Nicholson
  • J Stein
  • K A Wilson
چکیده

Mung bean plants (Wilczek) accumulate increasingly greater amounts of buffer-extractable copper in both their shoots and roots when grown in liquid medium containing greater than 2 micrograms per milliliter copper (31.4 micromolar) as cupric sulfate. This increase in soluble copper is accompanied by an increase in the relative amount of low molecular weight (7,000 to 20,000) macromolecular-bound copper and a decrease in the relative amount of high molecular weight (greater than 20,000) copper. The major low molecular weight copper protein has been isolated from copper-intoxicated mung bean plants by a combination of ammonium sulfate fractionation, gel filtration, and ion exchange chromatography. It was identified as mung bean plastocyanin on the basis of its molecular weight, optical behavior, and amino acid composition. No evidence was found for a low molecular weight copper-binding protein corresponding to mammalian thionein or chelatin.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Investigation of Plants Purification Capability of Cu on Two Cultivars of Mung Bean Plants in Contaminated Soils

Objective: To study plants purification capability of  Cu on two cultivars of vetch plant (Vigna Radiata) in contaminated soils and their effects on morphological characteristics. Methods: we conducted a completely randomized trial as factorial. Different concentrations of  Cu (0, 150, 300, and 400 mg/ Kg of dried soil) were used. After four weeks growth on contaminated soil, parameters such as...

متن کامل

Investigation of Plants Purification Capability of Cu on Two Cultivars of Mung Bean Plants in Contaminated Soils

Objective: To study plants purification capability of  Cu on two cultivars of vetch plant (Vigna Radiata) in contaminated soils and their effects on morphological characteristics. Methods: we conducted a completely randomized trial as factorial. Different concentrations of  Cu (0, 150, 300, and 400 mg/ Kg of dried soil) were used. After four weeks growth on contaminated soil, parameters such as...

متن کامل

Effect of Planting Pattern and Irrigation Method on Germination of Mung Bean (Vigna radiate) Harvested at Different Times of Maturation

DOR: 98.1000/2383-1251.1398.6.51.11.1. 1575.1578 Extended Abstract Introduction: Pulses are a group of crops which are important in human nutrition and also sustainability of agronomical systems and economic advantage. Regarding optimum planting density of mung beans (40 plant m-2), more than 700 tons of certified seeds of mung bean seeds are needed all over the country, confirming the im...

متن کامل

تأثیر گیاهان همراه سویا، لوبیا و ماش بر جذب کادمیم از خاک توسط ذرت و آفتابگردان به‌عنوان گیاه اصلی

In a pot experiment, the effect of soybeans, mung beans and beans on the corn and sunflowers in a cadmium contaminated soil was studied in a completely randomized design with three replications. The treatments consisted of intercropping of corn and mung bean corn and beans, corn and soybean, sunflower and mung bean, sunflower and beans, sunflower and soybean monoculture of corn, and monoculture...

متن کامل

Effect of Salinity and Osmotic Stresses on Some Economic Plants

The effect of water stress induced by NaCl or drought stress was studied on the leaves of wheat, barley, kidney bean and mung bean. After 25 days old, iso-osmotic stresses (200 mM) NaCl and PEG 6000 (180 g 1 ) were applied for 3 weeks old plants. Decreasing leaf dry weight was similar in -1 barley, kidney bean and mung bean under both stresses, while leaf dry weight of wheat decreased more unde...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Plant physiology

دوره 66 2  شماره 

صفحات  -

تاریخ انتشار 1980